Home About DDW Articles Contact
You can leave us your email address and we will keep you in touch with our news.
Deuterium-depleted water (DDW) inhibits the proliferation and migration of nasopharyngeal carcinoma cells in vitro

Authors: Hongqian Wang, Baohua Zhu, Zhiwei He, Hui Fu, Zhong Dai, Guoliang Huang, Binbin Li, Dongyun Qin, Xiaoyan Zhang, Lu Tian, Weiyi Fang, Huiling Yang
Published in: Biomedicine & Pharmacotherapy, Volume 67, Issue 6, July 2013, Pages 489-49
Recent studies have demonstrated that natural water that has 65% of the deuterium concentration depleted, can exhibit anti-tumor properties. However, the anti-tumor effects of DDW on various nasopharyngeal carcinoma (NPC) cells have not previously been reported. In the present study, NPC cell lines and normal preosteoblast MC3T3-E1 cells were grown in RPMI1640 media containing different deuterium concentrations (50- 150 ppm). The effects of DDW on the proliferation and migration of NPC and MC3T3-E1 cells were investigated using the MTT, plate colony formation, and Transwell assays, as well as Boyden chamber arrays, flow cytometry (FCM), western blot and immunofluorescence. We found that DDW was an effective inhibitor of NPC cell proliferation, plated colony formation, migration and invasion. In contrast, the growth of normal preosteoblast MC3T3-E1 cells was promoted when they were cultured in the presence of DDW. Cell cycle analysis revealed that DDW caused cell cycle arrest in the G1/S transition, reduced the number of cells in the S phase and significantly increased the population of cells in the G1 phase in NPC cells. Western blot analysis revealed that treatment with DDW significantly increased the expression of NADPH:quinone oxidoreductase-1 (NQO1), while immunofluorescence assay analysis revealed that treatment with DDW decreased the expression of PCNA and matrix metalloproteinase 9 (MMP9) in NPC cells. These results demonstrated that DDW is a novel, non-toxic adjuvant therapeutic agent that suppresses NPC cell proliferation, migration, and invasion by inducing the expression of NQO1 and causing cell cycle arrest, as well as decreasing PCNA and MMP9 expression.

» Trimite unui prieten  |  » Tipareste  | 


Alte Articole

» Living Water fights against seriouses diseases

» Water a source of health

» Further information on cancer treatment with deuterium depleted water

» Research concerning the radioprotective and immunostimulating effects of deuterium-depleted water.

» The effect of deuterium depleted water on cancerous cells

» In Search of the Fountain of Youth

» The living water

» Birocracy prevent patenting

» Deuterium depleted water and products based on it

» Living water against cancer

» Brandient created the brand QLARIVIA

» Where can you find Deuterium Depleted Water?

» Anti-aging Water Rewarded

» The Immaculate Water, in the top of research excellence

» A 100% Romanian product: the water from Valcea, deuterium depleted water, immaculate water or Qlarivia

» The water of life, unknown panacea

» The Living Water a 100% Romanian wonder: saves lives, delays aging

» Light water, the cure for heavy diseases

» Qlarivia - Immaculate Water for your wellness!

» Deuterium Depleted Water - what is it?

» Qlarivia, one of the best waters in the world

» We invite you to buy deuterium depleted water online

» Qlarivia = Slow aging?


» Deuterium-depleted water has stimulating effects on long-term memory in rats

» Deuterium content of water increases depression susceptibility:The potential role of a serotonin-related mechanism

» From the Japanese bathroom to the outer space

» Research studies unique in Europe and worldwide, conducted at ICSI Rm. Valcea

» We export hundreds of tons of living water

» What is deuterium and deuterium depleted water

» Local Exremum at 8.95 ?m in Spectrum of Mountain Water as indicator for Health and Longevity

» Studying of Isotopic Effects of Deuterium in Biological Objects

» In Vitro Antineoplastic Effects of Deuterium Depleted Water

My Story

Do you know cases of people who have benefited from the beneficial effects of deuterium depleted water? Do you have something to say about deuterium depleted water?
Write us your story and we will publish it on our website. Someone will surely have something to learn from your experience.
» Send us your story

DDW in World Press

» View all news